Verificación de la viabilidad de semillas de diferentes especies de orquídeas usando Tetrazolium

Abstract

Abstract: The storage of orchid seeds in banks is one of the most effective methods to conserve and propagate the species, therefore, in the present work the viability of some seeds was analyzed, which were stored in the seed bank of the UTPL at temperatures between4 to 10° C, for 10 to 12 years, for this the tetrazolium test was used, which consisted of immersingthe seeds in distilled water at 30° C for five days, after this period the water was replaced by tetrazolium and left to rest at 30° C for 24 hours, finally it is observed under the microscope;the viable seeds stain their embryo red, due to cellular oxidation, in addition many of these samples have a high percentage of vain seeds, which is an indication of the poor quality of these. Of the total samples analyzed, 13% of these showsgood viability, preserving 50% of viable seeds, among them are the Xylobium, Cyrtochilum, Anacheilium, Gongora, Helcia, Epidendrum and Cattleya genera.