Modelo computacional del metabolismo celular de yarrowia lipolytica con la incorporación de genes exógenos para la obtención de ácido docosahexaenoico

Abstract

Abstract: In the present work we evaluate the feasibility of taking advantage of the cellular metabolism of Y. lipolyticaas a biotechnological chassis for obtaining high added value chemicals from computational models. The metabolic model of Y. lipolyticainvolves 136 reactions and 128 metabolites and uses a carbon source for its growth. By means of Flow Balance Analysis and CNA in R, it was obtained afat acid production rate equal to 0.0640 mmol of DHA/gPS*h with a carbon load of 100 mmol and a biomass production rate of 0.2133 mg and per hour (mg/h) in wild type Y. lipolytica. By optimizing the production of DHA, knockout mutants were designed in three reactions of routes and pathways accessory to the original production pathway and as a result for the mutant within the oxidation pathway β with biomass data of 0.2409 mg/h and DHA of 0.2145 mmol/gPS*h . Our results validate the relevance of using Y. lipolyticafor the design of cell factories to obtain DHA on an industrial scale.