Cuantificación de vitamina e en especies arecaceas mediante cromatografía líquida en alta resolución acoplada a espectrometría de masas (HPLC-MS)

Abstract

Abstract: Vitamin E is one of the most powerful antioxidant agents, which helps to prevent deficiency diseases, its main source are vegetable oils rich in unsaturated fatty acids. This is why the objective of this work is to quantify vitamin E in threeArecaceae species from the province of Loja, using High Resolution Liquid Chromatography (HPLC). For this purpose, a procedure has beendetermined, which began with the species collection, the extraction of their oils through the Soxhlet method, the calculation of the yield and determination of physical properties. Later, with the development of the method for the injection of samples in HPLC coupled to UV-Visible and Mass Spectrometry; the results of the3 extracts were notfavorable as no compoundappearedin the chromatogram, therefore,itwas necessarythe use of controls (extra virgin olive oil and the skin of the bruise), obtaining new results andbeing able to identify isomers, such as,delta, gamma and alpha-tocopherol, verifying that the analytical method used was adequate and the studied samples donot have tocopherols.