Obtención de un consorcio con capacidad de producción de biohidrógeno mediante fermentación anaerobia, para el desarrollo de un proceso de revalorización de la biomasa de Eichhornia crassipes

Abstract

Abstract: Hydrogen can be obtained through anaerobic fermentation, which makes it possible to use vegetable biomass as raw material. This project seeks to isolate a microbial consortium to develop a hydrogen production process through anaerobic fermentation of Eichhornia crassipes residues. For this work cattle manure was subjected to successive transfers and heat shock to isolate hydrogen-producing heat-resistant acidogenic bacteria. Fermentations were carried out by measuring the consumption of sugars by DNS and the volume of hydrogen by displacement of water. Two culture media were used: with glucose and with Eichhornia crassipes hydrolyzed liquor. After 10 transfers there was a decrease in pH and an increase in gas volume with respect to the control. In the fermentations, a maximum of 240 and 20 mL of gas with glucose and hydrolyzed liquor were obtained respectively, the experimental data were compared with a theoretical analysis showing that it is possible to improve the yield. Eichhornia crassipes appears to be a promising alternative for producing hydrogen that requires improvements in plant hydrolysis and consortium adaptation.