Análisis químico y enantiomérico de la fracción volátil destilada de la especie Verbesina Arbórea de la provincia de Loja (Villonaco)

Abstract

Abstract: The Verbesina Arborea, is a native species of the Andean region of Ecuador, collected in the Province of Loja, in the Villonaco hill, 14 km from the City of Loja; The essential oil was obtained by steam distillation, in which leaves, stems and flowers were used; the chemical composition, enantiomeric and biological activity were determined. To determine its chemical composition and it was performed through gas chromatography coupled to CG / MS mass spectrometry, and CG / FID flame ionization detector, in addition to its chiral distribution; 58 compounds being identified that represent 92.76% of the total composition of the oil. The main compounds were the following: Phellandrene <-> (18.74%), Pinene <-> (8.74%), Sabinene (7.12%), Nonane <n-> (5.29%), Limonene (4.01%), Thujene <-> (2.98%). And after 25 minutes of retention, it is detected: Muurola-4 (14), 5-diene <cis-> (0.30%), Terpinyl acetate <&#945;-> (0.29%) and Germacrene B (0.09%); and the biological activity was carried out through the micro-dilution scheme in broth and in turn the physical properties of the oil were determined, which was presented as inactive oil for the strains studied.