Extracción y estudio del aceite esencial de Siparuna Aspera

Abstract

Abstract: In the present investigation, the extraction and study of the essential oil of Siparuna aspera was carried out. The collection of the species was carried out at the Chamusquín Station located on the Loja to Zamora Road. For oil extraction, the steam distillation method was used. The essential oil yield of this species was 0.4 ml/Kg. The chemical composition of the essential oil was determined by gas chromatography coupled to a mass spectrometer (GC-MS) and gas chromatography equipped with a flame ionization detector (GC-FID). In the oil, 27 compounds were identified that correspond to 100% of the identified percentage. The compounds found in the highest proportion were: α-pinene (19,46%), β-pinene (12,38%), β-mycene (5,79%), limonene (3.41%), (E)-Caryophyllene (9,21%), 2-(tridecanone 11,11%), Humulene epoxideII(6.04%),7-epi-α-(Eudesmol 6,95%),α-copaene (3,70%). The enantiomeric distribution and the enantiomeric excess wereperformed using a chiral column. Four pairs of enantiomers were identified: α-pinene, β-pinene, limonene, and germacrene D.