Determinar la actividad biológica del aceite esencial de Annona cherimola

Abstract

Abstract: Annona cherimola, the extraction of this plant was by distillation of the fresh leaves. The chemical composition of the essential oil (EO) was performed with a non-polar and a polar chromatographic column and the enantiomeric distribution with an enantioselective column. Qualitative analyses were determined by gas chromatography coupled to a mass spectrometer and quantitative analyses by gas chromatography coupled to a flame ionization detector. Antibacterial activity of EO and its major constituents were performed on gram-negative and one gram-positive bacteria. Radical properties of EO were performed by ABTS and DPPH assays. Spectrophotometric methods were used to measure acetylcholinesterase inhibitory activity. Chemical analysis identified fifty-three compounds, the main ones being germacrene D (28.77 ± 3.80%), β-pinene (4, 7.93 ± 0.685), bicyclogermacrene (11.12 ± 1.39%), (E)-caryophyllene (10.52 ± 1.64%) and sabinene (3, 9.05 ± 1.69%). EC exhibited strong activity against Klebsiella pneumoniae and Campylobacter jejuni with a MIC of 500 μg/mL and very strong anticholinesterase activity with IC50 of 41.51 ± 1.02 μg/mL.