Análisis de las propiedades bioactivas de una fracción polifenólica de piel de mango

Abstract

Abstract: Mango peel, traditionally considered a byproduct of industrial processing, contains mangiferin, a phenolic compound with antioxidant, anti-inflammatory, antidiabetic, and neuroprotective properties. In this study, an efficient method for purifying mangiferin from mango peel was developed using ultrasound-assisted extraction with 50% ethanol, followed by solvent removal and mangiferin isolation via flash chromatography, with its presence confirmed by HPLC. Ethanol concentrations ranging from 13% to 15%, a flow rate of 10 mL/min, a collection time from minute 50 to 112 were used, and the detection limit was set at 0.03 AU. Antioxidant properties were evaluated using DPPH, FRAP, and ABTS, resulting in a mangiferin-rich fraction with a 90.22% yield, an antioxidant activity IC50 of 18 μg/mL, and an AAI of 4.00 ± 0.03 μg/mL-1, indicating potent antioxidant effects. Additionally, its anti-acetylcholinesterase activity at 33.333 μg/mL, equivalent to 100 ppm of donepezil, was analyzed. These findings highlight the potential of mango peel as a valuable resource for industrial and pharmaceutical applications.