Análisis de las propiedades bioactivas de una fracción polifenólica de semilla de aguacate

Abstract

Abstract: Alzheimer's disease affects 4% of people over the age of 75. It is mainly treated with donepezil, which relieves symptoms but has side effects such as nausea and dizziness due to its synthetic origin, so effective alternatives are needed. Avocado seeds, a by-product that makes up 30% of the weight of the fruit, are typically discarded. To increase shelf life, the seeds were dried at 70°C for 13 hours. Extraction was performed with 31% ethanol using ultrasound, a rotary evaporator and a freeze dryer. Using high performance liquid chromatography, it was found that the ethanolic extract obtained a higher concentration of antioxidant activity, which was fractionated by flash chromatography. They were separated into four different fractions, identifying that fraction D (15-20%) has a higher antioxidant capacity 7789 ± 141 (DPPH) and 213 ± 6 mg GAE/g (TPC), which is characterized mainly by peak 50. Fraction B shows higher anti-acetylcholinesterase capacity in peak 30. This is the first study in which the compounds responsible for antioxidant and anti-acetylcholinesterase activity are fractionated and determined.