Crecimiento, consumo y producción de hidrógeno con Clostridium acetobutylicum a partir de fructosa como fuente de carbono y energí

Abstract

Abstract: In this study, the growth, consumption, and production of hydrogen were evaluated by Clostridium acetobutylicum using fructose as a carbon and energy source. Hydrogen was obtained through biological processes based on anaerobic fermentation. For this purpose, RCM culture medium was prepared with three fructose concentrations (1, 2.5, and 5 g L-1). Each flask was filled with a working volume of 30 mL and inoculated with the equivalent of 10% V/V. During fermentation, parameters such as gas production, cell growth, and sugar consumption were measured. Based on the kinetic characterization, the results showed that the bacteria were able to metabolize fructose; however, the experimental hydrogen yield was lower than theoretical, ranging from 0.870 mol H2 mol fructose-1 for the 1 g L concentration; 0.936 mol H2 mol fructose-1 for the second concentration; and 0.638 mol H2 mol fructose-1 for the third concentration. The highest volumetric hydrogen production was achieved at the 2.5 g L concentration, with a volume of 1.73 10-2 L L-1 h-1 of hydrogen.