Evaluación de un Aditivo Crioprotector Penetrante para la Crioconservación de Enterobacterales productoras de betalactamasas de espectro extendido del cepario del laboratorio de Microbiología de la Investigación de la Facultad de Ciencias de la Salud de la Universidad Técnica Particular de Loja

Abstract

Abstract: The main objective of this Curricular Integration Project (CIP) is to evaluate a penetrating cryoprotectant additive for the cryopreservation of extended-spectrum beta-lactamase-producing Enterobacterales (ESBL). To this end, eight bacterial strains were preserved with glycerol at three different concentrations: 10%, 15%, and 20%. Viability was then monitored using serial dilutions and colony-forming unit (CFU) counting to determine the recovery level of the cryopreserved strains. The results obtained from the evaluation of the cryoprotective additive at different concentrations revealed that the optimal percentage for cryopreservation of beta-lactamase-producing enterobacteriaceae (ESBL) is 10% glycerol, as it allows the bacteria to be cryopreserved while maintaining their viability, purity, and morphological stability. This research allowed us to identify the most appropriate glycerol concentration to preserve cell viability, thus optimizing cryopreservation, as well as validating the effectiveness of the method used. This study contributes to the development of effective techniques for the preservation of clinically important microorganisms and paves the way for future research in the field of bacterial resistance.