Desarrollo de un protocolo para la extracción de Benzo[a]pireno en sustratos de cultivo de past

Abstract

Abstract:Benzo(a)pyrene (B(a)p) is a persistent and highly toxic environmental contaminant. In order to optimize its extraction from solid matrices, a protocol was developed for its recovery in grass growing substrates. Experimental conditions were evaluated using the Soxhlet extraction system, a hexane:acetone mixture (98:2 v/v) as solvent, and a temperature of 70°C. The extractions were performed on contaminated samples in a controlled manner and were subjected to extraction times of 4 and 6 hours. The results showed a higher recovery of B(a)p with increasing extraction time, reaching yields of 96.7% and 92.7% at 6 hours, compared to 82.1% and 80.1% obtained at 4 hours. Quantification was performed using gas chromatography/mass spectrometry (GC/MS) using chrysene-D12 as the internal standard. It is concluded that the protocol is effective, reproducible, and feasible for application in various environmental studies, especially for the quantification of B(a)p in solid samples.