Criopreservación a -20°C y -80°C de hongos Ascomycetes en diferentes crioprotectantes.

Abstract

Ascomycetes fungi cryopreservation is essential in today's world, as the potential of these microorganisms is marked both biotechnology and research, hence the importance of well-preserved collections that meet the premises of a good process. The aim of this study was to evaluate the feasibility of ten species of fungi Ascomycetes cryopreserved at -20°C, -80°C and between -20°C and -80°C. The method required an incubation crioprotectantes, followed by slow thawing process in a water bath at 37°C in order to prevent cell damage by water crystallization. Finally they were placed in culture medium MEA to evaluate the potential of regrowth. It was determined that glycerol at -80°C is best suited for this group of fungi cryoprotectant addition it was possible to obtain important information in a convenient way cryopreserved other species of Ascomycetes fungal and other groups that are important to science.