Caracterizacion molecular y conservación de comunidades microbianas asociadas a la rizósferas de especies vegetales acumuladoras de metales pesados en suelos contaminados del sector Chinapintza, Zamora- Chinchipe

Abstract

Abstract: Microorganisms are a clear example of the enormous biodiversity contained in the soil. It proceeded to the in vitro isolation, molecular characterization and identification of microorganism's rhizosphere soil associated with Miconia zamorensis and Erato polymnoides, regarded as accumulators of heavy metals in the mining area of Chinapintza Zamora Chinchipe. The strains were identified by sequencing the 16S ribosomal RNA gene in bacteria, and identified the presence of genera such as Bacillus, Serratia and Lysinibacillus. In fungi was used the sequence of ITS ribosomal RNA gene and it was identified the genres Aspergillus, Talaromyces, Penicillium, Trichoderma, Nodulisporium, Fusarium, Rhizopus, Mucor, Hypochnicium and Umbelopsis. Such genres form part of the soil and are considered as tolerant to ecosystems with heavy metals contamination. Due to the high demand to elaborate collections of microorganisms for bioremediation purposes, it was proposed the cryopreservation of bacterial and fungal pure cultures, for that glycerol was used as cryoprotectant. It was obtained a 100% of viability in reviving bacterial cultures, while most of the fungal cultures were reactivated successfully.