Extracción, aislamiento y caracterización de metabolitos secundarios del extracto en diclorometano de la especie Loricaria thuyoides (Lam.) Sch. Bip.

Abstract

The leaves of Loricaria thuyoides knowed as "Trencillo de cerro", were collected in Saraguro town and its extraction, isolation and characterization of secondary metabolites was performed by spectroscopic techniques NMR and GC-MS. Two coumarinic compounds were identified known as scopoletin and, 5-hydroxy obliquin. Additionally, the isolation of two compounds of terpenic origin (referred as KE43-27 and KE194-73) and a mixture of fatty acids was achieved. The cholinesterases inhibitory activity of the compounds were evaluated by the ability to inhibit acetylcholinesterase and butyrylcholinesterase. The results evidenced that compound KE43-27 showed a significant inhibitory activity against acetylcholinesterase and butyrylcholinesterase with concentrations of 3.06 and 3.42 mU/ml respectively. While the compound 5-hydroxy-obliquin presented only inhibitory activity to butyrylcholinesterase (18.4 mU/ml) , and the compound KE194-73 resulted non active for enzymes tested.