Determinación de la actividad antimicrobiana de los estractos totales de cuatro especies vegetales de la provincia de Loja y Zamora Chinchipe : piper escuadorense 8 matico), lepechinia mutica Benth ( Turuyante), fuschia ayavancensis ( pena-pena), niphogeton dissecta ( culantrillo del cerro), empleando los métodos de difusión de placa, concentración mínima inhibitoria e inhibición del crecimiento radial

Abstract

Abstract: methanolic extracts of four plant species collected from the Province of Loja and Zamora Chinchipe: Piper ecuadorense (Matico), Lepechinia mutica Benth (Turuyante), Fuschia ayavacensis (Pena-Pena) and Niphogeton dissecta (Culantrillo del cerro), traditionally used in the treatment of many conditions including digestive disorders, respiratory, anti-inflammatories, and so on. To this study were used methods which permit to measure the in vitro susceptibility of microorganisms front to naturally substances with antimicrobial potential such as diffusion technique in agar-well, mycelial growing inhibition and broth microdilution front of S. aureus, E. faecalis, E. coli, P. vulgaris, P. aeruginosa, K. pneumoniae, C. albicans, S. typhimurium, S. cerevisiae, A. niger, T. mentagrophytes, T. rubrum using different concentrations in the methanolic extracts to determine their inhibition. In tests of antibacterial activity the results show the L. mutica species is more active against the S. aureus, P. vulgaris, E. faecalis, while the other extracts did not show any activity against the microorganisms tested in the spent dosage. In the antifungal test the species with more antifungal potential against the dermatophytes T. mentagrophytes and T. rubrum were: P. ecuadorense and N. dissecta to a concentration of 1000 µg/ml with an inhibition percentage of 100-96.78% respectively, following of a species L. mutica inhibiting 78-76% respectively, while the F. ayavacensis species shows less active against the dermatophytes inhibiting the 50% in growing. The obtained results allow us to determine the vegetable species home a potential application as antimicrobials due to we suggest to measure their properties in order to establish its usage as therapeutic agents.