DMSO como Control para Ensayos in vitro de Cierre de Herida en Cultivos Celulares humanos de próstata.

Abstract

Abstract: Dimethyl sulfoxide (DMSO) is a molecule with polar properties, used widely due to its activity as a solvent. Given its penetration properties of membrane and water displacement, its use is widespread in pharmacology and studies cells, although its toxicity is apparently low at concentrations <10%, it is rarely they inform. The present study is focused on evaluating the behavior of the lines PNT2 and PC3 cells exposed to different DMSO concentrations in the wound assay. For this purpose, viability and scratch wound closure tests were carried out at concentrations of 0.5, 5 and 10mM. An increase in the viability of the PNT2 cell line was observed at 48 hours, while for PC3 no differences were observed compared to the control without treatment. In the wound closure assay, the cell lines evaluated showed no differences significant, so they can be considered as a control for future trials.