Conservación de microorganismos a largo plazo

Abstract

Abstract: Microorganisms play a crucial role in our environment. Currently, the use of beneficial microorganisms has led to process improvements in various fields such as pharmaceuticals, industry, mining, and agriculture. Consequently, there is a need to preserve microorganisms of interest with potential applications in research. The objective of this study was to evaluate several methods for the long-term conservation of microorganisms. To achieve this, pure colonies were isolated from the banana rhizosphere. These colonies were morphologically characterized and preserved using the encapsulation method. Subsequently, they were subjected to four different temperatures: room temperature, 4°C, -24°C, and -80°C. Viability analysis was conducted at two, four, and five months. As a result, 10 pure bacterial isolates were obtained from bananas. These isolates were molecularly characterized based on the partial 16S region. The sequences were compared with the NCBI database, revealing the following genera: Bacillus, Enterobacter, Burkholderia, Cupriavidus, and Pandorea. Furthermore, statistical analysis indicated that the four temperatures do not significantly affect the viability of Bacillus, Burkholderia, and Cupriavidus bacteria. Similarly, for Enterobacter, room temperature does not impact its viability.