Actividad genotóxica y antigenotóxica mediante el análisis de micronúcleos de la horchata expendida en los mercados de Loja

Abstract

To the south of Ecuador in the province of Loja the consumption of the traditional drink called "horchata" is maintained that is elaborated from 16 to 32 plants and nowadays they not only sell it in markets of the province but they commercialize it in different presentations. Previous studies indicate that 3 different horchatas (HRCH2, HRCH7, HRCH8) of 9 lyophilized had a cytotoxic effect on brain astrocytoma tumor cells and by genotypic and antigenotoxic damage were measured by comet assay. The objective of the study was to evaluate its possible genotoxic and / or antigenotoxic effect of these mixtures at a concentration of 1000μg / mL in CHO-K1 cells by the micronucleus test with blockade of cytokinesis. It was demonstrated that the lyophilizates did not have a genotoxic effect, all presented an antigenotoxic effect demmutágeno, nevertheless like bioantimutágeno agent HRCH7 maintains the protective effect whereas HRCH2 and HRCH8 presented a diminished effect. None of the tested horchatas modulate the cellular proliferation, evaluated in the index of cellular division.