Efecto de la composición de un consorcio microbiano formado por microorganismos acidófilos Leptospirillum ferrooxidans y Acidothiobacillus thiooxidans sobre su adherencia en un mineral aurífero refractario.

Abstract

The bacterial adhesion on the surface of the mineral is one of the most important aspects in the processes of biooxidation of sulfides. Reason for which in the present work the formation of a microbial consortium was made determining its adherence to a mineral. The microorganisms used in the investigation were: Leptospirillum ferrooxidans DSM2705T and Acidothiobacillus thiooxidans DSM14887T which grew at 30 ° C and pH 1.8, were previously adapted to different concentrations of galactose and iron, proceeded to perform cell adhesion at different time intervals (1, 10, 30, 60, 120, 180, 240 min). Protein and number of adhered cells were determined. For the formation of the microbial consortium the proportions of each microbial species were varied in the following relationships 30/70; 40/60; 50/50; 60/40; 70/30% v / v of Leptospirillum ferrooxidans and Acidothiobacillus thiooxidans. The highest adherence of the culture A. thiooxidans was given using 0.15% of galactose, for the culture of L. ferrooxidans was given at 27g / L of iron concentration, while for the consortium was given in 60% L. ferrooxidans and 40% A. thiooxidans v / v with 83% adherence.