Formulación y Evaluación de la actividad in vitro de una crema antifúngica a partir del extracto de la Piper ecuadorense.

Abstract

Abstract:The present work was design to elaborate an antifungal formulation that consist to active the principle ethanolic extract, the formulation which were previously optimize with the experimental factorial design 23. The accelerated stability studies were carried out for the antifungal cream at the temperature conditions (environment, 30 ± 2°C and 40 ± 2 ° C at 70 ± 5% RH) were carried out during a period of (0 days, 30 days and 60 days), and also the concentration of the extract of Piper ecuadorense and the antifungal cream were evaluated by HPLC. The cream showed satisfactory results in terms of organoleptic properties, viscosity, pH and extensibility; likewise, with the microbiological tests, the safety of the cream was guarantee. The antifungal determination in vitro of the cream has been made by the technique of the microdilution in agar, against Trichophyton rubrum ATCC@28188 and Trichophyton mentagrophytes ATCC @ 9533, which showed to be active until a concentration of pinocembrin of 89.3% at 60 days at 30 ± 2°C. The study results demonstrate a 60-day stable cream, at three storage conditions, with a favorable pH for normal skin that fluctuates between 5.62 and 5.69.