Estudio Fitoquímico de tubérculo de la especie Eriotheca ruizzi

Abstract

The species Eriotheca ruizii was collected, in El Naranjo-Paltas-Loja. The tuber was segmented into epidermis, white and brown cortex, white and brown pulp, due to maturation, obtaining extracts; using solvents: hexane, ethyl acetate and methanol, phytochemical screening was carried out to evaluate the presence of phytoconstituents. The extracts that showed the highest biological activity in human cancer cell lines, ethyl acetate epidermis and white methanol cortex were fractionated by open column chromatography, and alkaloid extraction was performed to the epidermis methanol extract. Obtaining a mixture of 25 compounds of which 6 could be alkaloids and 6 others were identified as: palmitic acid methyl ester, heptadecanoic acid methyl ester, 8,11-octadecadenoic acid methyl ester, 7-octadecenoic acid methyl ester, methyl ester of octadecenoic acid, 2-octyl-cyclopropanotetradecanoic acid methyl ester; of the white cortex extract a compound identified as arabinofuranosyl- (l "→ 6 ') - glucopyranosyl-4-hydroxy-3-methoxy-phenethyl was obtained. For compound identification, gas chromatography coupled to masses, ¹H-NMR and ¹³C-NMR, HMBC, HSQC, COZY, retention index, melting point, retention factor.