Determinación de grupo filogenético en cepas de Escherichia coli aisladas de vegetales listos para el consumo y empacados

Abstract

Abstract:The ETA affect socioeconomic development worldwide, the plants are exposed to large amounts of pollutants, they harbor microorganisms of the enteric type (Salmonella, Escherichia coli (strain O157: H7), among others). E. coli is a gram-negative bacillus that is part of the intestinal microbiota and because of its great variability tends to adapt easily to any environment and thus be the cause of several diseases. The objective of the present work was to determine the phylogenetic group in strains of E. coli isolated from ready-to-eat vegetables and packed, 28 strains were analyzed by PCR-multiplex technique and the following results were obtained A (21.4%) and B1 (39.3%), B2 (17.9%) D (21.4%) classifying them into 3 groups: 60.7% commensal strains, 39.3% extraintestinal pathogens and in any of the 4 phylogroups the intestinal pathotypes (ETEC), (EIEC), (EAEC) , (EPEC), (EHEC) and (ADEC). It is concluded that the main source of contamination for plants are the bacteria eliminated in the excretion of animals and with lower percentage of human fecal matter, in addition the phylogenetic distribution can have several factors not only for animal species.