Validación de un método analítico por Cromatografía Líquida de Alta Resolución acoplada a Espectrometría de Masas (HPLC-MS) para la determinación y cuantificación de carnosol obtenido de la especie Lepechinia mutica.

Abstract

Abstract:Through the development of an analytical method, the presence of carnosol in ethyl acetate extract obtained from the species Lepechinia mutica was quantitatively determined. The method used meets the established parameters of linearity (r2= 0.9995), limit of detection (38,71 ng/μl) and limit of quantification (58,91 ng/μl). Based on the repeatability and reproducibility analysis, it was possible to determine the accuracy of the test, according to the established norms, the results of the variation coefficient must be less than 10 and 15% respectively, a repeatability percentage of 0.97% was obtained, while for reproducibility was 0.97 and 0.94%. Accuracy was assessed by studying the recovery percentage, which was 93 to 112%; because each parameter meets the stipulated conditions, the quality of the method is assured, concluding that it is reliable and can be applied for further investigations.