Aislamiento, identificación y evaluación de la actividad biológica de los metabolitos secundarios de Clinopodium tomentosum.

Abstract

Abstract: In the present phytochemical study of the leaves of Clinopodium tomentosum, collected in Loja, Ecuador, through CC, NMR and HPLC-MS spectroscopic methods, and corroborated with literature data, it was possible to identify 5 compounds, three flavonoids known as 5-hydroxy-4',7- dimethoxyflavanone (1), Apigenin-4',7-dimethyl éter (2), Salvigenin 5-hydroxy- 4', 6, 7- trimethoxyflavone (3), and a mixture of two structural isomers known as oleanolic acid (4) and ursolic acid (5). The bacterial activity was evaluated through MIC of the total extracts, the ethyl acetate extract submitted moderate activity against Micrococcus luteus (500 g / mL), the hexanic and methanol extract did not submit antibacterial activity. The antioxidant activity was evaluated by DPPH showed that the methanolic extract had a higher antioxidant activity, while the extract in hexane and ethyl acetate had a lower activity.