Aislamiento e identificación de enterobacterias patógenas presentes en vegetales frescos que se expenden en los supermercados de la ciudad de Loja.

Abstract

Abstract:The family enterobacteriaceae or also called enteric bacteria are part of the intestinal microbiota, are gram-negative bacilli that inhabit preferentially in the large intestine of people and animals, are characterized mainly because they are mobile, do not ferment lactose and are gas producers, although they are not pathogenic, it can cause infections such as Escherichia coli, which affects patients with weakened immune systems. The objective set out in this research was the identification of enterobacteria in fresh vegetables such as lettuce, cilantro, parsley, chard and spinach, which are sold in the main supermarkets of the city of Loja. This investigation consisted in the analysis of 165 samples that was carried out using the methodology proposed by the BAM, where it was started with the obtaining and preparation of the samples, pre-enrichment phase, enrichment in selective media, later it was isolated in solid media for your biochemical identification The presence of several organisms that correspond to the family of enterobacteria that are indicators of food contamination were detected: Escherichia coli 13, 33%, Citrobacter freundii 15.15% Pantoea agglomerans 10.30% Enterobacter aerogenes 6.66%.