Aislamiento e identificación de enterobacterias patógenas presentes en vegetales frescos provenientes de venta libre

Abstract

Abstract:Foodborne diseases are a public health problem worldwide, due to the intake of food contaminated with microorganisms or chemical substances / toxins produced by them. The objective of the present study was to isolate and confirm the presence of enterobacteria in fresh over-the-counter vegetables, considering the relevance of the pathogens belonging to this family in the development of diseases, fresh vegetables tend to be vulnerable due to the absence or little cooking that they require prior to their consumption. In 300 units of samples of lettuce, spinach, Swiss chard, parsley and cilantro, the methodology proposed by the Bacteriological Analytical Manual (BAM) was applied. The results obtained determined the presence of pathogenic enterobacteria such as Escherichia coli in 29.3%, presumptive Salmonella spp in 0.6% and presumptive Shigella spp presuntiva 1%, among other enterobacteria indicative of fecal contamination (Citrobacter freundii, Enterobacter aerogenes, Pantoea agglomerans, Enterobacter cloacae, Shigella spp, Klebsiella oxytoca, Klebsiella pneumoniae, Salmonella spp., Proteus mirabilis, Serratia marcescens), spinach was the vegetable with the highest contamination of Escherichia coli with 28.4% followed by parsley with a 22, 7%.