Validación de métodos analíticos para la determinación de Ca, Ba, Mg, Se, y Ni por absorción atómica en las matrices de agua residual, natural y potable

Abstract

Abstract:The validation of analytical methods for the determination of (Ba, Ca, Mg, Se and Ni) by atomic absorption spectrophotometry in the matrices of wastewater, natural and potable, begins with the revision of standardized methods, for metals in waters. The validation is carried out to comply with the requirements of the ISO / IEC standard: 17025, INEN 1108, Standard Methods23 edition, Eurachem Guide2016.The methodology used was the air-acetylene flame and nitrous oxide acetylene method with Perkin Elmer 400 Atomic Absorption Spectrophotometer. To guarantee the validation process, the following requirements were analyzed: working range, linearity of the method, limit of detection, limit of quantification, accuracy, uncertainty and precision.It was determined through statistical analysis that the different criteria for each metallic analyte were met, among these we have: veracity (80% <% R <120%), linearity presenting a correlation coefficient (R2) greater than 0.995, and finally an uncertainty (&#8804; 30% (K = 2)) at all concentration levels. Once the methods under study have been validated, the protocols for each method and a validation report are prepared.