Identificación molecular de Staphylococcus aureus meticilino resistente (SARM) de origen hospitalario

Abstract

Abstract: Staphylococcus aureus, a Gram positive bacterium, during the last decades has developed resistance patterns, highlighting among the most important resistance to Methicillin (MRSA), which can be classified into CO-MRSA (Community origin MRSA) and HA -SARM (MRSA of hospital origin), the latter is associated with nosocomial infections, such as endocarditis and skin conditions. For its identification, the multiplex PCR molecular method was used to determine the chromosomal cassettes (CC) SCCmec I, II and III, and the diffusion disk phenotypic method to establish the existence of the agr II gene. Of 49 (100%) samples analyzed, it was determined that 27 (55.1%) belong to HA-SARM and 3 (6.12%) do not have an established origin, because they share HA-SARM and CO-SARM genes; furthermore, it was found to identify that 2 (7.40%) samples contained the agr II gene.