Aislamiento del metabolito secundario geranyl acetate del aceite esencial de la especie Melaleuca armillaris.

Abstract

Abstract: The present research aims to determine the physical and chemical composition of the essential oil of the species Melaleuca armillaris, as well as the isolation and identification of secondary metabolite geranyl acetate by column chromatography (CC). In the essential oil (AE) by gas chromatography coupled to mass spectrometry (GC-MS) was able to identify 38 compounds that give a total of 99,92% of the oil, among the main compounds found were: 1,8-cineole (66,51%), α-Terpineol (8,83%), Limonene (10,34%), α-Pinene (5,23%), Myrcene (2,22%) and β-Pinene (1,55%).The density of the EO was 0,907 g/cm3, refractive index 1,474 g/cm3, optical activity +3,577°. A fractionation column of 4.5 ml of essential oil resulted in 3 mg of the compound geranyl acetate, identified by nuclear magnetic resonance (NMR of protons 1H and carbon 13C) and GC-MS. The essential oil and geranyl acetate did not exhibit inhibitory activity against the enzymes butyrylcholinesterase (BuChE) and acetylcholinesterase (AChE)