Citotoxicidad inducida in vitro por el compuesto 4,4''-[(3,4-dihidroxifenil)metilen]bis(3-metil-1-fenil-1H-pirazol-5-ol) en combinación con quimioterapéuticos sobre la línea celular RKO

Abstract

Abstract: Cancer is considered one of the diseases that causes the most deaths worldwide, which is why experts in this area look for new treatments for this pathology every day, and it depends on the type and stage, the type of therapy is assigned. At present, various drugs with cytotoxic activity have been developed that induce apoptosis in cancer cells; Such is the case of the use of pyrazoles as a treatment, these pyrazoles are aromatic compounds and when studied it has been shown that new anticancer agents can be developed from their chemical nucleus. In the present work, the cytotoxic activity of the compound 4,4 '' - [(3,4-dihydroxyphenyl) methylene] bis (3-methyl-1-phenyl-1H-pyrazole- 5-ol) or 3i in the colorectal cancer tumor cell line RKO and the normal prostate cell line PNT2, likewise the compound was combined with three chemotherapeutic drugs: 5-FU, MTX and MMC, at five different concentrations. The results demonstrated a greater cytotoxic effect of compound 3i on RKO cells compared to the normal cell line PNT2; while in the combinations carried out in RKO it is observed that there is a synergism of the cytotoxic effect up to the concentration of 10 µM and an antagonistic effect in the higher concentrations; on the other hand, in PNT2 a significant synergistic effect is not observed in the combinations made.