Estandarización de protocolos en el sistema de inmersión temporal RITA® para la producción de plantas in vitro de Cinchona officinalis y Coffea arabica

Abstract

Abstract: Cinchona officinalis, a species endemic to Loja, and Coffea arabica, a globally important crop, are species that can be massively multiplied using the RITA® temporary immersion system, optimizing their micropropagation and increasing their reproduction rate. Therefore, protocols for handling both species are being developed. Eight treatments were applied for each species, varying immersion times and different types of hormones for each species. Data were collected on contamination and phenolization; germination percentage and time; and number and length of seedlings and roots. The results of the T4 treatment with indole-3-butyric acid on Cinchona officinalis were the most effective, generating high root development and a multiplication rate of 1.16. For Coffea arabica, hormone-free treatments increased germination percentage (T9 and T13) and root length (T13), while the kinetin treatment with a two-minute immersion stimulated a greater number of roots. These SIT-RITA® protocols contribute to advances in plant biotechnology, facilitating the propagation of these highly valuable species.