Validación de método microbiológico cuantitativo recuento de E. coli en embutidos por método rápido petrifilm

Abstract

Abstract: This work validates the Petrifilm rapid microbiological method for quantifying Escherichia coli in sausages, analyzing parameters such as precision, accuracy, repeatability, limit of quantification, and uncertainty. The validation was carried out using AOAC method 991.14, which employs the direct plate count methodology for E. coli, using the ATCC reference strain #11775. The bacterial population was adjusted to different concentration levels (Levels I, II, and III) using a densitometer. The results show that the validation parameter values ​​are within acceptable ranges according to the Eurachem Guide, thus confirming the reliability of the results. The Petrifilm method represents an efficient alternative for quality control, thus contributing to food safety. Its implementation can be especially valuable in an environment where speed and accuracy are crucial to ensuring safe products for consumption.