Crecimiento, consumo y producción de hidrógeno con Clostridium acetobutylicum usando una mezcla de glucosa y xilosa como fuente de carbono y energí

Abstract

Abstract:The purpose of this study was to determine the biohydrogen yield obtained after anaerobic fermentation using a degenerate Clostridium acetobutylicum microorganism, using a mixture of glucose and xylose in different proportions as the carbon and energy source to achieve a lower-cost substrate. Three glucose/xylose ratios (70/30 50/50 30/70) were used, with an adaptation period for each concentration of five successive transfers every 24 h. Experiments were conducted in 50 mL airtight flasks with a working volume of 30 mL. The total gas volume was measured using a graduated syringe, and the H2 volume was calculated considering 50% production. When changing substrates, it can be observed that the mixture of substrates can be metabolized by the microorganism, but the biohydrogen yield from Clostridium acetobutylicum decreases. However, the bacteria exhibited greater cell growth. Thus, a yield of 0.7188 mol H2/mol of sugar, 0.4564 mol H2/mol of sugar, and 0.4877 mol H2/mol of sugar was reported for the 70/30 50/50 30/70 glucose/xylose ratios, respectively.