Determinación de las propiedades físicas, composición química y evaluación de la actividad biológica del aceite esencial de la hoja de Bixa orellana de la provincia de Zamora Chinchipe

Abstract

The leaves of the species Bixa Orellana were collected in the province of Zamora Chinchipe, in the canton Yacuambi, in the parish of La Paz,. From this species the essential oil was obtained by the steam distillation method, the determination of the chemical composition of the essential oil was performed by gas chromatography coupled to mass spectrometry CG-MS. To determine the antimicrobial activity of the essential oil the microdilution method was applied in broth and for the antioxidant activity the methods ABTS + and DPPH were used. The physical properties of the essential oil were also determined. The essential oil composition consists mainly of epi-α-Bisabolol, α-Santalene; nerolidol, α-Bergamotene, β-Farnesene, β-Caryophyllene and β-Santalene, presented a yield of 0.16%; Has a density of 0.92 g/cm3 ; Refractive index of 1.38 and optical activity of +12.64°. The essential oil presented antimicrobial activity against Micrococcus luteus, in addition presented antioxidant activity in the two methods evaluated, specifically in ABTS +.