Cuantificación del contenido de papaína y su actividad enzimática en seis especies del género Vanconcellea nativas del sur del Ecuador

Abstract

Abstract: The Enzymatic Activity of the enzyme papain of six species of the gender Vasconcellea was quantified, gatheredes in Loja and Zamora Chinchipe provinces. The species studied were: V. monoica, V. palandensis, V. stipulata, V. x heilbornii nm. var. Guisaguiña, V.cundinamarcensis, V. x heilbornii nm. var. Gualel. For the obtaining of this enzyme, the latex of the green fruits of the species studied was extracted and transported under freezing conditions and the separation of the same one was carried out for centrifugation to 13400 rpm for 30 minutes. Purified enzyme was added Bisulfite of Sodium to 1 % p/p for its conservation. The Enzymatic Activity was quantified through a reaction colorimetric measure in a Spectrophotometer UV, in a wavelength of 280 nanometres, applying the method (9001-73-4) AOAC 54. The results demonstrated that the Vasconcellea palandensis had a high yield in latex with a value of 1,40 % p/p; the biggest yield in the purified enzyme was the Vasconcellea x heilbornii nm. var. Gualel with a value of 34,11 % p/p and the Enzymatic Activity of the purified enzyme was bigger in the Vasconcellea stipulata with a value of 13097,20 USP/mg. After the drying for liophilization the Enzymatic Activity of this enzyme reached a value of 61281,00 USP/mg. The Enzymatic Activity of the enzyme papain lyophilized of the Carica papaya var. Tocaimera obtained under the same conditions, got a value of 33766,12 USP/mg, meaningly smaller that the enzyme lyophilized of the Vasconcellea stipulata.