Validación de métodos analíticos para la determinación de metales pesados ( cu, fe, zn, mn y pb), mediante absorción atómica en las matrices de aguas residuales, naturales y de consumo humano en los laboratoriosUTPL

Abstract

Abstract:The Validation of analytical methods for the determination of heavy metals (Cu, Fe, Mn, Zn and Pb) by atomic absorption spectrophotometer in matrices residual, natural and human consumption begins with a review of standardized methods for heavy metals presents in water. The validation is done to perform the requirements of ISO / IEC 17025, Standard Methods, Eurachem Guide. The methodology used was the method of air-acetylene flame, with Atomic Absorption Spectrophotometer Perkin Elmer 400. To ensure the validation process, the following requirements were analyzed: working range, linearity of the method, detection limit, quantification limit, accuracy, precision and uncertainty. It is determined by statistical analysis meet the various criteria for each metal analyst, among these are: accuracy (80% <% R <120%), linearity showing a correlation coefficient (r²) It is greater than 0.995 and finally an uncertainty (<= 30% (K = 2) ) at all concentration levels.