Determinación de las propiedades físicas, composición química, evaluación de la actividad biológica y antioxidante del aceite esencial del fruto Copal (Dacryodes peruviana) de la provincia de Zamora Chinchipe.

Abstract

The essential oil from the Dacryodes peruviana fruit was obtained through hydrodistillation. The chemical composition is determined by gas chromatography coupled with mass spectrophotometry and by flame ionization detector. Biological activity was evaluated by broth microdilution method and the antioxidant activity by two spectrophotometric methods: ABTS+ and DPPH. Oil physical properties were also determined 44 compounds components were identified representing 98,37% of the essential oil. The most representative components were: α-Phellandrene (50,32%), Limonene (23,03%), β-Phellandrene (8,27%), Terpinolene (5,23%), p-Cymene (3,06%), β- Pinene (2,57%) and Sabinene (1,44%).The density was 0,846 g/cm3, refractive index was 1,4751 and optical activity +12,154. The essential oil presented a weak biological activity in front of Staphylococcus aureus with a MIC of 0,625mg/mL, while other bacterial were inactive. The essential oil for this species did not present antioxidant activity for the different evaluated concentrations.