Caracterización morfo-molecular de hongos anamorfos del cepario UTPL y evaluación de su potencial antibacteriano.

Abstract

Secondary metabolites isolated from fungi, show promising antibacterial activity that could solve problems of clinical interest. However, very few studies have focused on the search or characterization of fungi out to date. This study attempts morphological (microscopic features) and molecular (nrDNA ITS-5.8S) characterization of anamorphic fungi, as well as obtaining extracts to assess their ability to inhibit seven pathogenic bacteria of clinical interest, by measuring the Minimum inhibitory concentration. We found that our 18 fungal cultures belong to the phyla Basidiomycota, Ascomycota, and Zygomycota. In the antagonism tests Pycnoporus sanguineus was active against Staphylococcus aureus, Escherichia coli, Proteus vulgaris and Salmonella typhimurium, while Clonostachys sp. Pyrenochaeptosis microspore and were active against Staphylococcus aureus. When assessing the extracts obtained from fungi found to be positive in the antagonism tests, it was determined that the inhibitory capacity was negative to the highest dose tested (MIC>1000 ug/mL). These results indicate that some fungi have antibacterial activity at high concentrations which are difficult to use as antibiotics for therapeutic use.