Determinación in vitro de la actividad antimicrobiana de los fármacos utilizados en el tratamiento de la mastitis bovina en el cantón Centinela del Cóndor de la provincia de Zamora Chinchipe.

Abstract

Livestock activity in cantón Centinela del Condor is widely represented by bovine production (milk and meat) through extensive exploitation. Moreover, within the area of this study and as one of the zoosanitary problems bovine mastitis (clinically and subclinically) can be found. The objective of this work is to isolate and identify those pathogens causing masitis and to determine antimicrobial activity against 14 antibiotics. Samples were collected at 18 bovine explotations from cantón Centinela del Condor. Milk samples were cultured in MacConkey, Salt Mannitol, and Blood Agar at 37°C for 24h. After growth, cultures were Gram stained and Catalase test to identify each bacterial genus. To determine which antibiotic was the most effective, inhibition zone was measured. The most representative bacteria were: Staphylococcus aureus (31,5%), Staphylococcus sp (16,6%), Staphylococcus catalase positive (16,0%), Shigella sp (15,5%), Escherichia coli (7.4%), and Streptococcus sp. (1,9%). Moreover, the most efficient antibiotics for the treatment of mastitis were: Bacitracin; Gentamicin, Kanamycin, Neomycin, Trimethoprim/Sulfamethoxazole and Tetracycline.