Aislamiento e identificación de metabolitos secundarios de la especie vegetal Solanum oblongifolium Dunal, en la provincia de Loja

Abstract

In the present research the leaves of the medicinal plant Solanum oblogifolium Dunal commonly known as "Turpe or Mataperros", harvested in the Saraguro canton belonging to the Province of Loja were used. From 500 g of dry sample was worked up by static maceration with a mixture of 8: 2 MeOH-H 2 O solvents, resulting in a total extract of 10.72 g.

A dechlorophyllation process was carried out by column chromatography (CC) from 5 g of total extract, obtaining a total of 2.37 g of extract of chlorophyll, which was subjected to liquid-liquid partitioning process using DCM, resulting in As the final product a DCM extract and an aqueous extract.

By column chromatography the two extracts were fractionated, 0.92 g of DCM extract and 1.15 g of aqueous extract, the fractions obtained were subjected to a TLC analysis for their respective binding according to the similarity of compounds and characterization by spectroscopic NMR techniques And CG-MS. Identifying a coumarin known as scopoletin and a flavonoid identified as isoquercitrin.