Aislamiento e identificación de metabolitos secundarios de la especie vegetal Solanum oblongifolium Dunal, en la provincia de Loja

Abstract

Abstract: In the present investigation the leaves of the medicinal plant Solanum oblongifolium Dunal commonly known as "Turpe or Mataperros", collected in the parish San Lucas, Cantón, Province of Loja were used. From the same that the extraction, isolation and characterization of secondary metabolites were carried out by means of chromatographic and spectroscopic techniques such as NMR and GC-MS. It was worked out from 500 g of dry sample of which by means of static maceration and rotaevaporation the total extract was obtained, to the same one that was carried out a liquid chlorination and liquid fractionation next using dichloromethane, obtaining two extracts an aqueous and one of DCM. Based on the richness of compounds and separation thereof, given by thin layer chromatography using solvents in proportions and different polarity for each extract, the conditions were fixed for fractionation by column chromatography, the resulting fractions were purified by microcolumns and analyzed by TLC. Finally two compounds of phenolic nature were identified, a flavonoid known as isoquercitrin resulting from the fractionation of the aqueous extract and a coumarin characterized as scopoletin, product of column fractionation of DCM extract.