Evaluación de actividad antimicrobiana de aceite esencial de achiote (Bixa orellana) para aplicaciones en modelos alimentarios

Abstract

Abstract: This thesis addresses the evaluation of antimicrobial activity of essential oil of Bixa Orellana in which 10 microorganisms were analyzed, including 3 gram bacteria. positive Staphylococcus epidermis, Staphylococcus aureus and Listeria monocytogenes, 5 gram negative bacteria such as Salmonella enterica subsp.enterica, Escherichia coli, Klebsiella aerogenes, Pseudomonas aeruginosa, Campylobacter jejuni, an Aspergillus mold niger and a Candida Albicans yeast; The methodology used for this analysis was the triplicate diffusion method in which the revivification of strains in which the revivification agar corresponding to each microorganism was used with its proper incubation conditions and the second fragment is the test of microbial resistance using a turbidity of 0.5 in each culture medium TSB, BHI, CYM corresponding to each microorganism and placing 2 dilutions in each petri dish of the essential oil (1:1; 1:3), the pure oil, a positive blank and a negative. The analysis of the antimicrobial evaluation was complemented with the Fisher statistical study (ANOVA), which I study the analysis of variance between the results obtained to observe the differences significant between dilutions and bacteria. In which the EO had antimicrobial activity against to the 3 gram positive bacteria, because their structure is much weaker, therefore Therefore, it could be considered that Bixa Orellana's AE would only have a reaction with gram positive bacteria.