Validación de método microbiológico cuantitativo aerobios mesófilos por método tradicional y rápido petrifilm en matriz de harina

Abstract

Abstract: In the present investigation, two microbiological methods were validated to determine mesophilic aerobes by the petrifilm AOAC 990.12 method and agar method FDA BAM C3 in a flour matrix. Parameters proposed by the guide were used Eurachem 2014 and ISO 17025: limit of detection, limit of quantification, reproducibility, repeatability, accuracy, precision. The samples were inoculated with strains certified at 3 concentration levels low, medium and high and were incubated for 24 h at 35°C, subsequently the counts were made in CFU/g. The data was analyzed and It was demonstrated that the validation parameters are within what is stipulated in the Eurachem guide especially with regard to the uncertainty parameter that was lower 20 on all three levels. With this validation it was demonstrated that the methods are repeatable and reproducible, increasing confidence in the validity of test results in food's microbiology.