Estudio de la composición química y actividad biológica del aceite esencial Siparuna muricata de la familia de las Siparunaceae en la Provincia de Loja

Abstract

This research is the study of the essential oil of S. muricata was obtained by distillation by steam, the average humidity was 62% and yield of 0.19%, the average density was 0.93 g/cm3 and the refractive index of 1.5000. We identified a total of 45 compounds in the essential oil corresponding 87.83% and 85.80% for DB5-MS and HP-INNOWAX respectively: germacrene D (41.79%), germacrene B (9.58%), delta cadinene (5.42% are the majority compounds in DB5-MS, and in the column HP-INNOWAX the majority compounds were: germacrene D (34.86%), germacrene B (7.99%), delta cadinene (6.16%), alpha guaiene (5.90%). Biological activity was determined by the broth microdilution method. The essential oil of Siparuna muricata in the first collection was inhibitory activity against the gram-positive Staphylococcus aureus (ATCC 25923), is obtained starting) with a concentration of 62.5 µg/mL, in the same way, the second and third collection showed inhibitory activity in the fungi Trichophyton mentagrophytes (ATCC 28185) and Trichophyton rubrum (ATCC 28188) with a concentration of 62.5 µg/mL and 125 µg/mL.