Evaluación de la susceptibilidad antimicrobiana y mecanismos de resistencia en cepas de Escherichia coli aisladas de vegetales frescos

Abstract

Abstract:Escherichia coli is a Gram negative, facultative anaerobic bacillus, present in the intestine of animals and humans, as well as in soils, it is considered as a fundamental member of the normal microbiota of the intestine. It has now become the enterobacteria of greatest concern in the food industry, because it causes severe gastrointestinal disorders in humans, mainly in children and the elderly. The objective of the project was to evaluate antimicrobial susceptibility and resistance mechanisms in strains of E. coli. analyzing a total of 144 samples. A 45.71% of strains of E. coli were isolated, and 54.29% of strains classified as fecal coliforms. Bacterial susceptibility was evaluated by means of the disc diffusion technique against 14 antibiotics and the evaluation of the mechanisms of resistance to β-lactams and non-β-lactams. A low percentage of resistance to therapeutic antibiotics such as tetracycline was evidenced. , streptomycin and trimethoprim sulfamethozazole; and, the sensitivity of more than 90% for ceftazidime, cefepime, imipenem and amikacin. So, with these results, ESBL-producing strains were not found.