Obtencion de extractos, aislamiento y caracterización de metabolitos secundarios de la especie Erythrina smithiana

Abstract

The species Erythrina smithiana, belonging to the family Fabaceae was gathered in the neighborhood Landangui, belonging to Malacatos's parish of the province of Loja, of the air parts extracts were obtained in Hex, AcOEt and MeOH, in order to isolate and to identify metabolitos secondary. The extract that mostro major performance was that of MeOH with 4.96 %. All the obtained extracts were analyzed by chromatography in column using for his diverse solvent elución in ascending polarity, of Hexano's extract a pure compound identified as Escualeno was isolated, and 6 mixtures of alcanos, triterpenos and acids that on having been analyzed by Gas chromatography connected to masses might be: mixes 1: Heneicosano, Octacosano, Docosano, Nonacosano and Heptacosano; mixes 2: Octadecano, Nonacosano, Hexacosanol, Heptadecano and Hentriacontano; mixes 3: 24 (S)-Ethyl-3-alpha, 5-alpha cycle cholest-22 (E)-en-6-ona and Estigmasterol-4-en-3-ona; mixes 4: acetate of β-amirina and Lupeol; mixture 5 isolated several secondary metabolitos but the only one that was possible his identification was Fitol. Of the extract of Acetate of Ethyl a mixture, mixture identified 7: Acid 1,2-Benzenedicarboxilico and 1-Heneicosanol. For the elucidation of these secondary metabolitos spectroscopic technologies were used and espectrométricas as: RMN 1H, 13C, and CG/EM.