Uso del lactosuero para la producción de bioetanol

Abstract

In the present project of investigation, the objective was the obtain bioethanol from permeate dairy serum and determine the optimal conditions for the fermentation process. For this, It has been made previously a enzymatic hydrolysis process, using the enzyme β-galactosidase to unfold the lactose present in the dairy serum in its two sugars (glucose and galactose), and then the fermentation using the yeast Saccharomyces cerevisiae. The fermentation was performed out at temperatures of: 20, 30 and 35°C, and times of 48, 72 and 120 hours and then a simple distillation it was done. The variables of temperature process and time were analyzed in MINITAB 16 where was obtained, a p-value of 0.042 therefore exists a significant effect between these two factors. According to the statistical analysis, the optimal conditions for fermentation were based on the decrease of °Brix, 20°C for 48 hours with 2.1°Brix, and according to the higher alcoholic strength content 20°C for 120 hours with 1.15°GL.